FIG. 6.

Representative Western blots (A) and summary data (B) of phospho-AKT Ser473 and phospho-AKT Thr308 protein expression after incubation of cultured trophoblast cells with control media, insulin (1 nmol/l), gAd (5 μg/ml), or fAd (5 μg/ml) for 24 h. Subsets of cells were pretreated with insulin (1 nmol/l) for 4 h and then exposed to 5 μg/ml gAd (IgAd) or fAd (IfAd) for an additional 20 h. n = 5 placentas for each treatment. Insulin significantly increased expression of both phospho-AKT Ser473 (P < 0.01) and Thr308 (P < 0.01). Addition of fAd to insulin-stimulated cells significantly (P < 0.01) reduced expression of phospho-AKT Thr308 compared with insulin treatment alone. Tukey-Kramer multiple comparisons post tests, *P < 0.05, **P < 0.01.