TABLE 3.

Fold difference in mRNA levels of genes regulating inflammation, metabolism, tissue remodeling, and antigen presentation in eATMΦ subtypes after 12 weeks of HFD relative to benchmark M2-polarized MGL1⁺/CD11c⁻ eATMΦs from mice fed an LFD for 8 weeks

	8 weeks LFD	12 weeks HFD
	MGL1+ CD11c−	MGL1+ CD11c−	MGL1med CD11c+	MGL1− CD11c+
Pro-/anti- Inflammatory markers
Gene
IL-13	*	8.15 ± 3.09†	2.08 ± 0.66*	0.15 ± 0.11‡
Ym-1	*	7.98 ± 2.16†	1.04 ± 0.18*	0.68 ± 0.17*
STAT6	*	5.24 ± 1.78†	1.51 ± 0.25*	0.86 ± 0.20*
SPHK1	*	0.52 ± 0.08†	0.41 ± 0.05†	0.22 ± 0.02‡
IL-10	*	1.32 ± 0.11†	0.62 ± 0.12‡	0.75 ± 0.20*‡
IL-1Ra	*	2.94 ± 0.35†	8.47 ± 1.56‡	7.40 ± 1.01‡
CD163	*	0.28 ± 0.01†	0.13 ± 0.006‡	0.02 ± 0.005‡
CD206	*	0.57 ± 0.12†	0.38 ± 0.03†	0.22 ± 0.02‡
Clec-7a	*	1.05 ± 0.18*	1.36 ± 0.20*†	1.84 ± 0.19†
IL-1β	*	1.15 ± 0.12*	0.95 ± 0.17*	1.99 ± 0.21†
Caspase-1	*	0.57 ± 0.04*	0.54 ± 0.05*	0.52 ± 0.05*
IL-12p40	*	4.85 ± 1.24†	6.80 ± 1.61†	11.4 ± 3.53†
TLR-4	*	0.91 ± 0.09*	0.89 ± 0.09*	0.56 ± 0.05†
MCP-1	*	0.37 ± 0.10†	0.21 ± 0.02†	0.03 ± 0.004‡
Metabolism
Genes
ARG-1	*	0.70 ± 0.13*	0.93 ± 0.17*	0.98 ± 0.12*
iNOS	*	0.53 ± 0.25*	0.09 ± 0.01†	0.03 ± 0.01‡
PGC-1α	*	43.7 ± 13.6†	283.4 ± 88.5‡	107.8 ± 24.2‡
PGC-1β	*	0.56 ± 0.07†	0.56 ± 0.13†	0.53 ± 0.14†
ADRP	*	1.75 ± 0.14†	2.26 ± 0.30†	2.24 ± 0.26†
Remodeling
Genes
MMP-2	*	4.95 ± 0.91†	6.39 ± 1.90†	1.66 ± 0.48*
MMP-12	*	40.7 ± 10.3†	360 ± 55‡	292 ± 46‡
TGFβ-1	*	2.43 ± 0.35†	2.36 ± 0.49†	2.41 ± 0.42†
VEGF	*	2.48 ± 0.17†	5.48 ± 1.17‡	8.95 ± 2.53‡
ADAM-8	*	6.18 ± 0.38†	14.28 ± 0.82‡	14.57 ± 0.77‡
LYVE-1	*	0.42 ± 0.15†	0.26 ± 0.08†	0.005 ± 0.001‡
Antigen presentation
Genes
I-a	*‡	0.71 ± 0.08*	0.46 ± 0.08†	0.99 ± 0.07‡
CD80	*	0.37 ± 0.04†	0.26 ± 0.04‡	0.18 ± 0.04‡
CD86	*	0.48 ± 0.05†	0.17 ± 0.03‡	0.10 ± 0.02‡

Data are means ± SE. Means designated by different symbols are significantly different (P < 0.05, ANOVA and Tukey procedure).