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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: FEBS Lett. 2002 Sep 11;527(1-3):199–204. doi: 10.1016/s0014-5793(02)03229-5

Fig. 2.

Fig. 2

Relative molecular mass (Mr) of native Ae-HKT/AGT and Dm-Spat. The Mr of native Ae-HKT/AGT and Dm-AGT was assessed by gel filtration chromatography on a Pharmacia Superdex 200 HR gel filtration column (1×30 cm) with thyroglobulin (670 000), γ-globulin (158 000), ovalbumin (44 000) and myoglobin (17 000) (Bio-Rad) as molecular mass standards. The mobile phase consisted of 20 mM phosphate buffer (pH 7.0) containing 150 mM NaCl at a flow rate of 0.2 ml per minute. Under the applied chromatographic conditions, the retention times of thyroglobulin (F), γ-globulin (E), Ae-HKT/AGT (D), Dm-Spat (C), ovalbumin (B), and myoglobin (A) were 44.3, 58.8, 60.0, 65.3, 77.4 and 84.0 min, respectively.