Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: FEBS Lett. 2002 Sep 11;527(1-3):199–204. doi: 10.1016/s0014-5793(02)03229-5

Table 2.

Cosubstrate specificity of Ae-HKT/AGT

Keto acid 3-HK Kynurenne
Pyruvate 100 100
Glyoxylate 123 78
p-Hydroxypyruvate 3 2
Indo-3-pyruvate 1 2
α-Ketoadipate ND ND
α-Ketobutyrate 22 26
α-Ketoglutarate ND ND
α-Ketovalerate 5 7
α-Keto-γ-methiolbutyrate 12 17
Methy-oxobutanoic acid ND ND
Oxaloacetate 177 92
Phenylpyruvate 33 42

Purified recombinant Ae-HKT/AGT was incubated in the presence of 3-HK or kynurenine and different amino group acceptors (α-keto acids) as described in Section 2. Data are expressed as a percentage of the activity of that with pyruvate as the amino acceptor and are the means of two separate experiments. Under the applied conditions, the specific activities of recombinant Ae-HKT/AGT towards 3-HK and kynurenine were 38 and 17 µmol/min/mg protein, respectively. ND: no detectable activity.