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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Biotechnol. 2010 Mar 14;28(4):348–353. doi: 10.1038/nbt.1612

Figure 2.

Figure 2

Figure 2

3D pinpointing methods of V3D.

(a) 3D pinpointing using 2 mouse-clicks. The color image is a 3D confocal image of neurons, fluorescently tagged for three different transcriptional factors (repo, eve, and hb9) at the same time, in a fruit fly embryo. A and B: non-parallel rays generated at two viewing angles, corresponding to two mouse-clicks; p: the estimated 3D location that is closest to both A and B.

(b) 3D pinpointing using 1 mouse-click. p1 to pN: the progressively estimated centers of mass; R1 to RN: the progressively smaller intervals to estimate p1 to pN.