Fig. 3.

Protein–protein interaction network models for α₅β₁–FN and α₄β₁–VCAM-1 complexes. (A and B) Proteins identified in α₅β₁–FN (A) and α₄β₁–VCAM-1 (B) complexes after a subtractive proteomics strategy were mapped onto a human interactome to generate interaction network models. Proteins identified within two path lengths (the 2-hop neighborhood) of β₁ integrin are shown, with the 1- and 2-hop neighborhoods forming the inner and outer circles, respectively. Protein identifications (nodes) are colored according to their relative enrichment to α₅β₁–FN (red) or α₄β₁–VCAM-1 (blue) and are ordered in a clockwise fashion relating to their relative enrichment to α₅β₁–FN. Proteins identified in both α₅β₁–FN and α₄β₁–VCAM-1 datasets are indicated by a black node border; absence of a black border indicates unique identification in that integrin-ligand dataset.