OA-NO2 reduced foam cell formation in vivo and inhibited phosphorylation of STAT-1 in vitro as well as in vivo. A, Representative Oil Red O stained peritoneal macrophages harvested from apoE−/− mice after 4 weeks of treatment. OA-NO2 reduced intracellular lipid accumulation. B, OA-NO2 decreased oxLDL-induced phosphorylation of STAT-1 in BMDMs. One representative blot is shown. Densitometric analysis of 3 independent experiments (p=0.001, *p=0.02 and #p=0.006 vs. oxLDL). C, Phosphorylation of STAT-1 was also reduced in vivo as shown by immunofluorescence. Arrows indicate pSTAT-1 positive cells (Magnification 20X and 60X, scale bar indicates 100 µm; blue=nuclei, red=pSTAT-1).