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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Toxicol Sci. 2007 Apr 12;98(1):99–109. doi: 10.1093/toxsci/kfm085

FIG. 3.

FIG. 3

Protein concentration does not affect the persistence of [3H]TCDD binding to Hartley guinea pig hepatic cytosolic AhR. Guinea pig hepatic cytosol of increasing protein concentrations (2–10 mg/ml) was incubated with [3H]TCDD in the absence or presence of 100-fold excess TCDF for 2 h. The final concentration of [3H]TCDD was 2nM for the 2 mg/ml samples, and 10nM for the 5 and 10 mg/ml samples. After 2 h, incubation were charcoal stripped to remove free and loosely bound ligand and then incubated with 200nM TCDF (2 mg/ml samples) or 1μM TCDF (5–10 mg/ml samples) at 20°C. Specific binding of [3H]TCDD was determined by the HAP assay at the indicated times. Data are presented as the mean ± SD of at least triplicate incubations.