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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Toxicol Sci. 2007 Apr 12;98(1):99–109. doi: 10.1093/toxsci/kfm085

FIG. 5.

FIG. 5

Persistent binding of [3H]TCDD and [3H]βNF to guinea pig hepatic cytosolic AhR is observed at 37°C. Guinea pig hepatic cytosol (2 mg/ml) was incubated with 2nM [3H]TCDD in the absence or presence of 200nM TCDF, or 10nM [3H]βNF in the absence or presence of 1μM TCDF for 2 h, charcoal stripped to remove free and loosely bound ligand and then incubated with TCDF (200nM or 1 μM, respectively, at 37°C). Specific binding of [3H]TCDD and [3H] βNF was determined by the HAP assay at the indicated times. Data are presented as the mean ± SD of at least triplicate incubations.