Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Toxicol Sci. 2007 Apr 12;98(1):99–109. doi: 10.1093/toxsci/kfm085

FIG. 6.

FIG. 6

[3H]E2 dissociates relatively rapidly from bovine uterine cytosolic ER. Bovine calf uterine cytosol (2 mg/ml) was incubated with 2nM [3H]E2 in the absence or presence of 200nM DES for 2 h, charcoal stripped to remove free and loosely bound ligand and incubated with cold DES (200nM) at 20°C. Specific binding of [3H]E2 was determined at the indicated time using the DCC method as described under the “Materials and methods” section. Data are presented as the mean ± SD of at least triplicate incubations.