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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: J Mol Biol. 2007 Mar 7;368(5):1426–1437. doi: 10.1016/j.jmb.2007.02.091

Figure 7. Contour diagram of the fraction of survival.

Figure 7

The fraction of survival (fs) at the end of the assay with thermolysin is determined with Eqs. 1 and 4 using kint of 8.8 s−1. kop and kcl are the forward and reverse rate constants for opening to the cleavable state. kcl is the intrinsic rate constant for proteolysis of proteins in the cleavable state. The kop and kcl values giving ΔGop of 0 kcal/mol and 9.0 kcal/mol are indicated with white solid lines. Cleavable states in the red region are accessible under the assay condition and proteins with a cleavable state in the red region cannot pass the survival assay. The white ‘×’ symbol indicates the location of the cleavable state of MBP through which this protein is digested by thermolysin.