Table 1. Identification of proteins from 2-Dimensional electrophoresis gels.
| A. Identified proteins from spots on the 2-D gel of E. coli lysate digested with 0.40 mg/mL trypsin for 4 days. | |||||
|---|---|---|---|---|---|
| Spot number | Protein ID | Gene name | Length | M.W. (kDa) | pI |
| 1 | P21179 | katE | 753 | 84.2 | 5.54 |
| 2 | P06715 | Gor | 450 | 48.8 | 5.64 |
| 3 | P76108 | ydcS | 359 | 40.0 | 6.27 |
| 4 | P06977 | gapA | 330 | 35.4 | 6.58 |
| 5 | P25887 | yghA | 294 | 31.4 | 6.32 |
| 6 | P60651 | speB | 306 | 33.6 | 5.14 |
| 7 | P24223 | pdxJ | 242 | 26.3 | 5.61 |
| 8* | P00882 | deoC | 259 | 27.7 | 5.50 |
| 9 | P04790 | tpiA | 255 | 27.0 | 5.64 |
| 10 | P02925 | rbsB | 271 | 28.5 | 5.99 |
| 11 | P09551 | argT | 238 | 25.8 | 5.22 |
| 12 | P12758 | udp | 252 | 27.0 | 5.81 |
| 13 | P12758 | udp | 252 | 27.0 | 5.81 |
| 14 | P75743 | ybgI | 247 | 26.9 | 5.07 |
| 15 | P21367 | ycaC | 208 | 23.1 | 5.20 |
| P32661 | rpe | 225 | 24.6 | 5.13 | |
| 16 | P12758 | udp | 252 | 27.0 | 5.81 |
| 17* | P12758 | udp | 252 | 27.0 | 5.81 |
| 18 | P09743 | deoD | 238 | 25.8 | 5.42 |
| 19 | P10344 | glnH | 226 | 25.0 | 6.87 |
| 20 | P00448 | sodA | 205 | 23.0 | 6.44 |
| 21 | P00448 | sodA | 205 | 23.0 | 6.44 |
| 22 | P10344 | glnH | 226 | 25.0 | 6.87 |
| 23 | P09157 | sodB | 192 | 21.1 | 5.58 |
| 24 | P09157 | sodB | 192 | 21.1 | 5.58 |
| 25* | P17288 | ppa | 175 | 19.6 | 5.03 |
| 26† | P04790 | tpiA | 255 | 27.0 | 5.64 |
| 27† | P12758 | udp | 252 | 27.0 | 5.81 |
| 28* | P11056 | bfr | 158 | 18.5 | 4.69 |
| 29† | P27430 | dps | 166 | 18.6 | 5.72 |
| 30 | P23827 | eco | 142 | 16.1 | 5.94 |
| B. Identified proteins from spots on the 2-D gel of E. coli lysate digested with 0.40 mg/mL thermolysin for 4 days. | |||||
|---|---|---|---|---|---|
| Spot number | Protein ID | Gene name | Length | M.W. (kDa) | pI |
| 1 | P21179 | katE | 753 | 84.2 | 5.54 |
| 2* | P07024 | ushA | 525 | 58.2 | 5.40 |
| 3* | P13482 | treA | 535 | 60.5 | 5.36 |
| 4 | P14178 | pykF | 470 | 50.7 | 5.77 |
| 5* | P23843 | oppA | 517 | 58.4 | 5.85 |
| 6 | P00391 | ipdA | 473 | 50.6 | 5.79 |
| 7 | P06715 | gor | 450 | 48.8 | 5.64 |
| 8* | P22259 | pckA | 540 | 59.6 | 5.46 |
| 9* | P19926 | agp | 391 | 43.6 | 5.38 |
| 10 | P37095 | pepB | 427 | 46.2 | 5.60 |
| 11* | P75691 | yahK | 349 | 38.0 | 5.80 |
| 12 | P00509 | aspC | 396 | 43.6 | 5.54 |
| 13 | P11665 | Pgk | 386 | 41.0 | 5.08 |
| 14 | P02928 | malE | 370 | 40.7 | 5.22 |
| 15 | P31133 | potF | 344 | 38.3 | 5.53 |
| 16 | P76108 | ydcS | 359 | 40.0 | 6.27 |
| 17* | P06977 | gapA | 330 | 35.4 | 6.58 |
| 18 | P13652 | cdd | 294 | 31.5 | 5.42 |
| 19† | P14178 | pykF | 470 | 50.7 | 5.77 |
| 20† | P14178 | pykF | 470 | 50.7 | 5.77 |
| 21 | P02925 | rbsB | 271 | 28.5 | 5.99 |
| 22 | P00882 | deoC | 259 | 27.7 | 5.50 |
| 23 | P09551 | argT | 238 | 25.8 | 5.22 |
| 24 | P30859 | artI | 224 | 25.0 | 5.32 |
| 25 | P04790 | tpiA | 255 | 27.0 | 5.64 |
| 26 | P32697 | aphA | 212 | 23.5 | 5.94 |
| 27 | P10344 | glnH | 226 | 25.0 | 6.87 |
| P30860 | artJ | 224 | 24.9 | 5.97 | |
| 28 | P10344 | glnH | 226 | 25.0 | 6.87 |
| 29 | P00448 | sodA | 205 | 23.0 | 6.44 |
| 30 | P12758 | udp | 252 | 27.0 | 5.81 |
| 31 | P21367 | ycaC | 208 | 23.1 | 5.20 |
| 32 | P32661 | rpe | 225 | 24.6 | 5.13 |
| 33 | P17288 | ppa | 175 | 19.6 | 5.03 |
| 34 | P09157 | sodB | 192 | 21.1 | 5.58 |
| 35† | P07651 | deoB | 407 | 44.4 | 5.11 |
| 36*† | P12758 | Udp | 252 | 27.0 | 5.81 |
| 37† | P12758 | Udp | 252 | 27.0 | 5.81 |
| 38 | P11056 | Bfr | 158 | 18.5 | 4.69 |
| 39 | P27430 | Dps | 166 | 18.6 | 5.72 |
| 40† | P05313 | aceA | 434 | 47.5 | 5.16 |
*
The spot intensity has been decreased noticeably compared with the same spot on the 2-D gel of the lysate digested for 1 day under the same condition.
†
The size of the peptide estimated from the gel suggests the spot corresponds to a fragment from proteolysis of the protein.