Fig. 2.
Identification of TNI-1 and UNC-27 as the major TNI isoforms in the myoepithelial sheath. (A) Western blot analysis of TNI and actin in total lysates from wild-type or unc-27-null worms that were treated with control RNAi, tni-1(RNAi) or tni-3(RNAi). (B-S) Wild-type (B-J) or unc-27-null (K-S) worms were treated with control RNAi (B-D and K-M), tni-1(RNAi) (E-G and N-P) or tni-3(RNAi) (H-J and Q-S), and dissected gonads were stained for TNI (left column) and F-actin (middle column). Merged images are shown in the right column (TNI in green and F-actin in red).
