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. 2010 Mar 16;11:13. doi: 10.1186/1471-2091-11-13

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Copyright ©2010 Chen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Purification of P. kullae K24 AzoB from the recombinant E. coli. Lane M, protein size markers; lane 1, extraction of the recombinant E. coli; lane 2, ammonium sulfate deposition suspension; lane 3, pooled fractions with phenyl Sepharose FF; lane 4, fraction of concentrated sample of the pooled fractions with phenyl Sepharose FF; lane 5, pooled fractions with HiPrep SP XL.