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. 2010 Mar 16;11:13. doi: 10.1186/1471-2091-11-13

Table 2.

Michaelis constants (Km) and maximal velocities (Vmax) of recombinant AzoB of P. kullae K24 expressed in E. coli.

Substrate Km (μM)* Vmax (U/mg protein)
NADPH 1.0 33
Orange I (NADPH) 3.0 36
NADH 170 17
Orange I (NADH) 8.6 22

* Data were presented by the averages from triplicate with standard deviations of <10%;