Figure 1.

The inhibition of formation of Reactive Oxygen Species (ROS) was evaluated in human polymorphonuclear (PMN) cells. The DCF-DA mean fluorescence intensity in the cells was proportional to the oxidative damage. Untreated cells served as a baseline for oxidative activity in the absence of oxidative stress. The presence of BC fractions MTB and CW led to inhibition of the spontaneous ROS production in the cultures (A). H₂O₂ was used to induce oxidative stress in the cultures. Cultures treated with H₂O₂ in the absence of GBC30 fractions served as a positive control for ROS formation. Cultures treated with GBC30 fractions MTB and CW in the presence of oxidative stress showed reduced ROS formation compared to cultures treated with H₂O₂ alone (B). Data reflect averages of cultures performed in quadruplicate for each test condition. The data shown are representative of three independent experiments performed on PMN cells from different donors.