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Primers for pyrosequencing analysis.
| Genes | PCR primers(5'-3') | Length (bp) | CpG site |
|---|---|---|---|
| Oct-4 | ATTTGGATGAGTTTTTAAGGGTTTT | 292 | 23 |
| ACTCCAACTTCTCCTTATCCAACTT | |||
| Nanog | TTTTTTAATTATAATTTGATGGGGT | 288 | 13 |
| CTAACACACCTTAAATAAACAAACC | |||
| Sox2 | GGGATATGATTAGTATGTATTTTTT | 230 | 15 |
| TTCTCCATACTATTTCTTACTATCCTCC | |||
