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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: J Infect Dis. 2010 May 15;201(10):1580–1588. doi: 10.1086/652005

Figure 3.

Figure 3

LTA associated with the surface (A), cell membrane (B) and culture supernatant (C) and adhesion to buccal cells (D) of wt M5 and its ΔNH2 mutant. Bacteria were grown overnight in THB in the absence and presence of SpeB inhibitor (E64), harvested and washed in distilled water to determine LTA content in the culture supernatant (naturally released LTA), trypsin extract (surface LTA) followed by phenol extract (membrane LTA) and adhesion to immobilized buccal cells by ELISA. Data are presented as the mean +/−S.D. from three experiments. The asterisks denote significant differences between wt and the ΔNH2 mutant values (p<0.01).