FIG. 2.
Phosphate release probabilities at various temperature values for the post-rigor, rigor-like, and pre-powerstroke states of Dicty myosin II. Release probabilities were evaluated by computing the fraction of Pi replicas released in fifty explicit-water MCES runs. Pi-release was judged on a distance criterion by using a cutoff of 30 Å from the initial position of the ligand. Uncertainties were estimated from the standard deviation of the probability distributions obtained at various temperatures of the ligand.