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. 2010 Apr 21;5(4):e10272. doi: 10.1371/journal.pone.0010272

Figure 10. Analysis of the effects of α-amanitin on regulatory factor recruitment.

Figure 10

Cells transfected with the indicated factors were pre-treated with α-amanitin for 2 hrs then incubated with tamoxifen for 3 hrs. (A) YFP-GCN5 (panels a–c) and YFP-Brd2 (panels d–f) were enriched at the transcription site marked by Cherry-tTA-ER. YFP-MS2 was not enriched at the transcription site after α-amanitin treatment (panels g–i) but did accumulate at the transcription site in untreated cells (panels j–l). Scale bar represents 5 µm. Scale bar in enlarged inset represents 1 µm. (B) Graph shows the percentage of cells with accumulations of each factor at the transcription site after α-amanitin pre-treatment followed by a 3 hr incubation with tamoxifen. 100 cells were analyzed from 3 independent experiments. SEM (in the form of error bars) are presented in the graphs.