Skip to main content
. 2010 Apr 21;5(4):e10262. doi: 10.1371/journal.pone.0010262

Figure 4. GPS NMR overview of apo BLA perturbed by addition of SDS, GdmCl, or TFE, by changes in pH, or heating.

Figure 4

GPS NMR 3D score plot (A) for titrations of apo BLA with SDS (0–56 mM; black), pH (pH 7.0–2.0; red), GdmCl (0–3 M; blue), TFE (0–50%; orange), TFE at pH 2.0 (0–50%; pink), and heating (283–333 K; green). SDS titrations at 700 and 400 are included. The lighter lines show projections on the PC1-PC3 plane. The scores of pure apo BLA and the A-state are indicated by a red dot and an italic A, respectively. Colored numbers indicate turning/end-points of the various titrations. (B, C) Overlays of NMR spectra of partially folded states (B) induced by 5 SDS molecules/protein (black), 1.6 M GdmCl (blue), and addition of acid to pH 2 (red), and (C) by 22 SDS/BLA (black), 50% TFE (orange), and 50% TFE at pH 2 (pink).