Figure 6. CD spectrum of I₅K₂ in MES buffer (pH 6.5).

The spectrum was measured at 25.0°C with 1 mm path-length. Concentration of peptide was adjusted to 0.55 mM in MES buffer (pH 6.5). The spectrum was recorded between 190 and 250 nm with 1 nm resolution and a 2 s averaging time. The final spectrum was background-corrected by subtracting the corresponding buffer spectra obtained under identical conditions.