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. 2010 Apr 21;5(4):e10189. doi: 10.1371/journal.pone.0010189

Figure 5. ecSOD localized in caveolae/lipid rafts via HBD enhances VEGF-induced VEGFR2 autophoshorylation in these microdomains.

Figure 5

A. After sucrose gradient centrifugation to isolate caveolae/lipid rafts, equal volume of each fraction from top to bottom (total 13 fractions) was IB with anti-VEGFR2, caveolin-1, or paxillin Abs (upper panel). In lower panel, Ad.LacZ or Ad.ecSOD or Ad.ecSOD-ΔHBD-infected HUVECs were used for caveolae/lipid rafts isolation, and each fraction was IB with anti-ecSOD or caveolin-1 Abs. B. Ad.LacZ or Ad.ecSOD-infected HUVECs were stimulated with VEGF (20 ng/ml) for 5 min, and followed by caveolae/lipid rafts fractionation. Equal amounts of proteins from pooled Fraction 4–6 (caveolae/lipid rafts) and Fraction 9–13 (non-caveolae/lipid rafts) were IB with anti-VEGFR2-pY1175, total VEGFR2 or caveolin-1 Abs (n = 3). *p<0.05.