Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Comp Biochem Physiol Part D Genomics Proteomics. 2008 Oct 17;4(1):21–31. doi: 10.1016/j.cbd.2008.09.003

Fig. 2.

Fig. 2

2D-DIGE analysis of proteomic changes induced by acute anoxia using an internal standard. (A) Schematic of experimental design in which three 2D gels were run with lysates from 24 hpf embryos using the 24 hpf, normoxic sample as the internal standard (Cy2, Cy3, Cy3 in gels 1, 4, and 5 respectively), and comparing 24 hpf + 1 h normoxia (Cy3, Cy5, Cy2) with 24 hpf + 1 h anoxia (Cy5, Cy2, Cy5). (B) Heat map analysis of statistically significant (p<0.05) proteomic changes after 1 h of anoxia. The color in each panel represents the relative change compared to the internal standard (24 hpf, normoxic) in each individual gel. (C) Image of Gel 4 indicating the location of the spots comprising the heat map.