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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Neurobiol Aging. 2008 Aug 12;31(6):993–1002. doi: 10.1016/j.neurobiolaging.2008.07.004

Table 1.

Oligonucleotides used in assays for DNA repair activities

Name bp Sequence
8-oxodG 30 5′-ATA TAC CGC G8C CGG CCG ATC AAG CTT ATT
graphic file with name nihms78206t1.jpg 3′-TAT ATG GCG CCG GCC GGC TAG TTC GAA TAA
dUracil 30 5′-ATA TAC CGC GUC CGG CCG ATC AAG CTT ATT
graphic file with name nihms78206t2.jpg 3′-TAT ATG GCG CGG GCC GGC TAG TTC GAA TAA
5-OHdU 30 5′-ATA TAC CGC G5C CGG CCG ATC AAG CTT ATT
graphic file with name nihms78206t3.jpg 3′-TAT ATG GCG CGG GCC GGC TAG TTC GAA TAA
5-OHdU bubble 51 5′-GCT TAG CTT GGA ATC GTA TCA TGT A5A CTC GTG TGC CGT GTA GAC CGT GCC
graphic file with name nihms78206t4.jpg 3′-CGA ATC GAA CCT TAG CAT AGG CAC CCG ACA AAC ACG GCA CAT CTG GCA CGG
AP 30 5′-ATA TAC CGC GGF CGG CCG ATC AAG CTT ATT
graphic file with name nihms78206t5.jpg 3′-TAT ATG GCG CCG GCC GGC TAG TTC GAA TAA

The modified bases are shown in bold letters. Underlined bases indicate unpaired area. bp: base pairs. (8) 8-oxodG; (U) dU; (5) 5-OHdU; (F) tetrahy-drofuran.