A) Representative photomicrographs of CREB (upper panel) and p-CREB (lower panel) gold-immunolabeling in CeA structures of P rats infused with aCSF or NPY during voluntary alcohol drinking. These photomicrographs show either CREB- or p-CREB-positive nuclei in the CeA of P rats infused with aCSF or NPY. The inset figures show the immuno-gold particles within a single nucleus (CREB, or p-CREB). Scale bar = 40 μm. B) Effect of NPY infusion into the CeA and voluntary alcohol drinking on CREB or p-CREB protein levels in CeA, MeA, and BLA of P rats. Values are mean ± S.E.M. of 7 rats per group (WW+aCSF, Water/Water + aCSF; WW+NPY, Water/Water + NPY; WE+aCSF, Water/Ethanol + aCSF; WE+NPY, Water/Ethanol + NPY). *Significantly different from the P (WW+ aCSF) rats (p < 0.001; one-way ANOVA followed by the Tukey test).