Skip to main content
. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Circ Cardiovasc Genet. 2010 Feb 2;3(2):169–178. doi: 10.1161/CIRCGENETICS.109.908905

Figure 3. Release of the GPIHBP1-C65Y mutant from the surface of cells with a phosphatidylinositol-specific phospholipase C (PIPLC).

Figure 3

CHO pgsA-745 cells were electroporated with empty vector, an expression vector for wild-type human GPIHBP1, or an expression vector for GPIHBP1-C65Y. 24 h later, the cells were incubated with PIPLC (5 U/ml) in serum-free medium for 1 h at 37° C. The amounts of GPIHBP1 in the cell culture media and the cell lysates were assessed by western blotting with a goat antibody against the S-protein tag. Actin was used as a loading control.