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. 2010 Apr 16;8:e003. doi: 10.1621/nrs.08003

Figure 1. Expression and transcriptional activities of DamK9A-hERα fusion protein.

Figure 1

(A). HeLa cells were transfected with various plasmids as indicated and cell lysate was analyzed by Western blot. MCF-7 and untransfected HeLa cell lysate were loaded as positive and negative control, respectively. The same blot was detected with anti-β-actin antibody as loading control. Molecular weights (Mw) are indicated as kilodaltons (kDa). (B). Fusion protein transcriptional activities in HeLa cells. Cells were co-transfected with various plasmids and luciferase reporter vectors. Luciferase activities were measured 24 hours after ligand treatment. Data are presented as mean +/- SEM.