Skip to main content
. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Cancer Res. 2007 May 15;67(10):4605–4619. doi: 10.1158/0008-5472.CAN-06-3619

Figure 5.

Figure 5

Testis-specific genes SYCP2 and TCAM1 are induced by HPV16. A, real-time qRT-PCR was done with total RNA extracted from NIKS cells with and without HPV16. Also, total RNA from NIKS-16 cells without HPV16 E7 protein expression was used to show that testis-specific gene induction by E7 protein was partial. B, SYCP2 induction in HPV+ cell lines were confirmed with Western blotting using anti-human SYCP2 antibody. Real-time qRT-PCR was done with total RNA extracted from primary cervical keratinocytes with either, or both, HPV16 E6 and E7 delivered by recombinant retrovirus. C, retrovirus without HPV16 gene was used as mock control. D, STAG3 mRNA expression in various cell lines was quantified using qRT-PCR and relative fold change to NIKS cells are plotted. Columns, mean; bars, SD.