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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: Cancer Res. 2006 Oct 1;66(19):9393–9400. doi: 10.1158/0008-5472.CAN-06-0984

Figure 1.

Figure 1

Early cervical phenotypes. Histologic cross sections of cervical epithelium from mice treated for 6 weeks with estrogen. Brown, positive staining; blue, hematoxylin counterstain. Dashed line, location of the basement membrane. A, pRb inactivation in murine cervix. pRb staining. B and C, BrdUrd incorporation in cervical epithelia. BrdUrd staining labels newly synthesized DNA. B, DNA synthesis is restricted to the lowest two cell layers in control epithelium, but E7 expression induces suprabasal DNA synthesis. Rb deletion in K14CreRbflox/flox cervix fails to reproduce the effect of E7. C, E7 fails to induce suprabasal DNA synthesis in the presence of the RbΔL allele. BrdUrd staining pattern in K14E7RbWT/WT cervix is indistinguishable from K14E7Rbflox/flox cervix (data not shown).