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. 2010 Jan 20;87(4):683–690. doi: 10.1189/jlb.0709513

Figure 2.

Figure 2.

Lentiviral constructs reduce ABCA1 and ABCG1 protein levels in human THP-1 cells. (A) Monocytes were transduced by lentivirus with shRNA for ABCA1. Protein was isolated after 7 days of puromycin selection (10 μg/ml), and Western blot analysis was performed. Data are presented as means ± sem (n=3). Results are representative of three experiments and compared with those in macrophages transduced with lentiviral scramble (P<0.0002). Immunoblot is shown below. (B) Monocytes were transduced by lentivirus with shRNA for ABCG1. Protein was isolated after 7 days of puromycin selection (10 μg/ml), and Western blot analysis was performed. Data are presented as means ± sem (n=3). Results are representative of three experiments and compared with those in macrophages transduced with lentiviral scramble (P<0.02). Immunoblot is shown below.