Figure 5.

A2AR agonist CGS-21680 decreases IFN-γ-stimulated foam cell formation in untreated and ABCG1 KD cells but not in ABCA1 KD cells. (A) THP-1 cells, ABCG1 KD cells, or ABCA1 KD cells were incubated with PMA (106 cells/ml) for 48 h. Cells were then incubated with acLDL (50 μg/ml) ± proinflammatory cytokine IFN-γ (500 U/ml), selective A2AR agonist CGS-21680 (1 μM/ml), and ± selective A2A antagonist ZM-241385 (1 μM/ml) for an additional 24 h. Foam cells were quantified and are expressed as a percentage of the total number of cells present in each chamber. wt, Wild-type. (B) Oil Red O stain was used to visualize lipid droplets in THP-1/macrophages.