Figure 3.

Liver CD11b⁺Gr1⁺ cells exhibit distinct phenotypic and morphologic properties. (A) Liver NPC were harvested from mice with i.p. MCA38 tumors (Day 17). Distinct CD11b⁺Gr1^high [high side-scatter (SSC)] and CD11b⁺Gr1^inter (low side-scatter) populations were expanded in the liver. Data are representative of experiments done more than five times using more than three mice in each experiment. FSC, Forward-scatter. (B) CD11b⁺Gr1^high or CD11b⁺Gr1^inter liver populations were FACS-sorted and then cytospun at high density (5×10⁶ cells/ml; upper panels) or low density (5×10⁵ cells/ml; lower panels), stained with H&E, and visualized by light microscopy (original, 20×). (C) Phenotypic analysis of Ly6C and Ly6G expression on bulk CD11b⁺Gr1⁺ cells from the spleen, liver, BM, or tumor in mice bearing advanced i.p. MCA38 tumors. Shaded histograms represent isotype controls. (D) CD11b⁺Gr1^inter cellular frequency in various tissue compartments of tumor-bearing hosts (P<0.05). (E) Expression of surface markers on hepatic CD11b⁺Gr1⁺ cells from mice with advanced MCA38 tumor after overnight culture. Shaded histograms represent isotype controls. MFIs are indicated below the histograms for each respective analysis. (F) Alterations in selected surface marker expression on hepatic CD11b⁺Gr1⁺ cells from C57BL/6 tumor-bearing mice after CFSE labeling and injection into CD45.1⁺ naïve mice. Data shown are representative of experiments repeated more than three times using three mice/data point. *, P < 0.05.