Table 2.

Hybridization data for Invader LNA probes and their free energy, $\mathrm{\Delta }{G}_{\text{rec}}^{293}$, for recognition of dsDNA target ON1:ON2 at 293 K.^a

Entry	ON	Duplex	Tm [ΔTm] (°C)	ΔH[ΔΔH] (kJ/mol)	(T293ΔS)[Δ(T293ΔS)] (kJ/mol)	ΔG293[ΔΔG293] (kJ/mol)	$\mathrm{\Delta }{G}_{\text{rec}}^{293}$ (kJ/mol)
1	ON3	5’-d(GGX ATA TAT AGG C)	36.5	−262	−214	−48	−26
	ON7	3’-d(CCA XAT ATA TCC G)	[−1.0]	[+120]	[+111]	[+9]
2	ON4	5’-d(GGT AXA TAT AGG C)	41.0	−360	−301	−60	−19
	ON8	3’-d(CCA TAX ATA TCC G)	[+3.5]	[+22]	[+24]	[−3]
3	ON5	5’-d(GGT ATA XAT AGG C)	40.5	−375	−315	−60	−22
	ON9	3’-d(CCA TAT AXA TCC G)	[+3.0]	[+7]	[+10]	[−3]
4	ON6	5’-d(GGT ATA TAX AGG C)	36.0	−311	−260	−51	−24
	ON10	3’-d(CCA TAT ATA XCC G)	[−1.5]	[+71]	[+65]	[+6]
5	ON11	5’-d(GGX AXA TAT AGG C)	48.0	−219	−165	−55	−34
	ON14	3’-d(CCA XAX ATA TCC G)	[+10.5]	[+163]	[+160]	[+2]
6	ON12	5’-d(GGX ATA XAT AGG C)	53.0	−209	−152	−56	−47
	ON15	3’-d(CCA XAT AXA TCC G)	[+15.5]	[+173]	[+173]	[+1]
7	ON13	5’-d(GGX ATA TAX AGG C)	48.0	−230	−175	−55	−50
	ON16	3’-d(CCA XAT ATA XCC G)	[+10.5]	[+152]	[+150]	[+2]

^aConditions as specified in Table 1.