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. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Org Biomol Chem. 2010 Mar 4;8(9):2028–2036. doi: 10.1039/b923465a

Table 3.

Kinetic parameters (t50%/t75% [min]) for recognition of isosequential dsDNA-target ON1:ON2 by selected Invader LNA.a

Buffer ON4:ON8 ON6:ON10 ON11:ON14 ON13:ON16
t50% t75% t50% t75% t50% t75% t50% t75%
MS 38 74 34 67 29 60 48 103
HS 126 257 - - 31 68 - -
KMST 158 296 - - 19 42 - -
a

[probe] = [target] = 1.0 µM; t50% and t75% = time in min required to reduce fluorescence intensity at λem = 495 nm by 50% and 75%, respectively. MS = medium salt, HS = high salt, KMST-buffer specified in main text