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. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Org Biomol Chem. 2010 Mar 4;8(9):2028–2036. doi: 10.1039/b923465a

Table 5.

Hybridization data for duplexes involving ON4 or ON8 and mismatched DNA.a

Entry ON Sequence Tm
[ΔTm]
(°C)		 ΔG293
[Δ(ΔG293)]
(kJ/mol)
1 ON17 5’-d(GGA ATA TAT AGG C) 39.5 −57
ON8 3’-d(CCA TAX ATA TCC G) [−9.0] [+13]
2 ON19 5’-d(GGT ATC TAT AGG C) 39.0 −58
ON8 3’-d(CCA TAX ATA TCC G) [−9.5] [+12]
3 ON21 5’-d(GGT ATG TAT AGG C) 44.5 −63
ON8 3’-d(CCA TAX ATA TCC G) [−4.0] [+7]
4 ON23 5’-d(GGT ATT TAT AGG C) 46.0 −64
ON8 3’-d(CCA TAX ATA TCC G) [−2.5] [+6]
5 ON25 5’-d(GGT ATA TAA AGG C) 37.0 −55
ON8 3’-d(CCA TAX ATA TCC G) [−11.5] [+15]
6 ON4 5’-d(GGT AXA TAT AGG C) 37.5 −54
ON18 3’-d(CCT TAT ATA TCC G) [−10.0] [+12]
7 ON4 5’-d(GGT AXA TAT AGG C) 38.0 −56
ON20 3’-d(CCA TAG ATA TCC G) [−9.5] [+10]
8 ON4 5’-d(GGT AX A TAT AGG C) 39.5 −58
ON22 3’-d(CCA TAC ATA TCC G) [−8.0] [+8]
9 ON4 5’-d(GGT AX A TAT AGG C) 35.0 −53
ON24 3’-d(CCA TAA ATA TCC G) [−12.5] [+13]
10 ON4 5’-d(GGT AXA TAT AGG C) 38.0 −56
ON26 3’-d(CCA TAT ATT TCC G) [−9.5] [+10]
a

Conditions as specified in Table 1. Underlined positions denote position of mismatched base pairs. ΔTm = change in Tm value and Δ(ΔG293) = difference in ΔG293 value, relative to fully matched ON1:ON8 (entries 1–5) or ON4:ON2 (entries 6–10).