Fig. 1.
Representative MRM chromatogram of SAL enantiomers and DA found in rat brain NAC (17 mg) spiked with (S)-, (R)-SAL-d4, and DA-d4 as internal standards. (S)- and (R)-SAL were found to be 0.9 and 1.0 pg / mg, respectively, and DA level was 4.5 ng / mg. The separation was achieved using a mobile phase changing from MeOH:IPA 8:2 to 3:7 in 5 minutes with the flow rate of 0.12 ml / min.