Fig. 4.

Calcitonin decreases calcitonin receptor expression in wild type osteoclasts but not in Crem knockout cultures. wide type and KO BMCs were treated with 30 ng/ml each of RANKL and M-CSF for 4 days to induce osteoclast formation, and then treated with vehicle (Veh) or 10 nM calcitonin (CT) for 5 h. Calcitonin receptor (CTR) expression relative to β-actin in wild type (WT) and Crem knockout (KO) osteoclasts treated with vehicle or calcitonin. Each value is the mean ± SD of 3 cultures. Different from vehicle-treated WT cultures, ** p<0.01.