FIG. 1.
Mutational analysis of the TGFβ1 responsive element of the α1(I) collagen promoter. EMSA was performed with 1 pg of the labeled Sp1.1 oligonucleotide and 8 μg of nuclear extracts form LX-2 human stellate cells. The arrowheads indicate the protein–DNA complexes formed. The boldface letters indicate the mutated sequences in each mutant. NS, nonspecific band; F, the free probe; TGFβ1, transforming growth factor β1; EMSA, electrophoretic mobility shift assay.
- WT: 5′-CTTCCCTCCTCCTCCCCCTCTCC-3′
- M1: 5′-CTTTTCTCCTCCTCCCCCTCTCC-3′
- M2: 5′-CTTCCTACCTCCTCCCCCTCTCC-3′
- M3: 5′-CTTCCCTTTTCCTCCCCCTCTCC-3′
- M4: 5′-CTTCCCTCCATCTCCCCCTCTCC-3′
- M5: 5′-CTTCCCTCCTTTACCCCCTCTCC-3′
- M6: 5′-CTTCCCTCCTCCTTTCCCTCTCC-3′
- M7: 5′-CTTCCCTCCTCCTCCTTCTCTCC-3′
- M8: 5′-CTTCCCTCCTCCTCCCCTACTCC-3′
- M9: 5′-CTTCCCTCCTCCTCCCCCTTACC-3′