FIG. 4.

EMSA with supershift showing binding of nuclear extracts form LX-2 stellate cells to the Sp1.1 oligonucleotide. The EMSAs were performed with 10 μg of nuclear extract and the labeled oligonucleotide. For supershifts the antibody (Ab) to cKrox (1:200 dilution) or preimmune serum (PI) was added to the reaction mixture. The arrowheads indicate the protein–DNA complexes formed. The arrows indicate the location of the supershifted complexes. NS indicates a nonspecific band; F indicates the position of the free probe.