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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Environ Mol Mutagen. 2009 May;50(4):317–327. doi: 10.1002/em.20462

Fig. 5.

Fig. 5

nDNA damage induced by MMS. Relative levels of amplification of a 6.9-kb nDNA fragment (from the PFK2 gene) in yeast cells treated with MMS. Yeast cells (A, wild type; B, apn1Δ mutant) were treated with increasing concentrations of MMS for 20 min, followed by DNA isolation. QPCR analysis was performed as described in the “Materials and Methods” section. (A, B) Representative gel electrophoresis indicating the expected sizes of the 6.9-kb nDNA fragment. Lanes 1–6: PCR products from yeast cells treated with 0, 0.025, 0.05, 0.075, 0.1, 0.15% MMS, respectively; (C) Relative levels of amplification of the 6.9-kb nDNA fragment (n = 3 independent experiments performed in triplicate). Asterisks (*) denote statistical significance (P < 0.05). Error bars represent SEM.