Figure 6.

Increased expression of Tfr2 in 129/S and FVB mice fails to increase hepcidin levels. (A-B) The same protocols were used and measurements were made as in Figure 5, except AAV-Tfr2 was injected into wild-type FVB or wild-type 129/S mice. No significant changes in the iron parameters of the liver or serum, including Tf saturation, total serum iron (TSI), transferrin iron-binding capacity (TIBC), or hepcidin, were detected despite the higher Tfr2 mRNA levels and TfR2 protein levels in the AAV-Tfr2–injected mice (■) compared with the AAV-c–injected mice (▩). Four mice were used in each set of measurements. The experiment was repeated with 4 more mice in each group with similar results. Error bars represent SD.