Table 1.
Gene | Target | MO sequence |
---|---|---|
npm1a | ATG/5′UTR | TAATGTTATCCTCCATTTTTGCGCG |
npm1a | ATG/5′UTR 5-bp mismatch | TAATcTTATCgTCgATTTTaGCcCG |
npm1a | Exon2-intron2 splice donor (2)* | AAGAAACATCACATACCATTCTAAC |
npm1a | Exon3-intron3 splice donor | TTATGACCAAGTCTACTTACACTTG |
npm1b | ATG/5′ UTR | GACCCATCTGTTCGAGATCCATGTC |
npm1b | ATG/5′UTR 5-bp mismatch | GAgCCATgTGTTCGAcATCgATcTC |
npm1b | Exon2-intron2 splice donor | TCAAATATTCATCTTCCTCACCGAC |
p53 | ATG/5′UTR | GCGCCATTGCTTTGCAAGAATTG |
Std cont | Human β-globin intron mutation | CCTCTTACCTCAGTTACAATTTATA |
Bold letters indicate the ATG codon; lowercase letters, mismatched residues; and Std cont, standard control.
(2) is the 1 working morpholino of 2 tested (supplemental Figure 1F, available on the Blood Web site; see the Supplemental Materials link at the top of the online article).