Table 1. Summary of phenotypes for Smed-prep(RNAi) experiments.
Experiment | Nr. Exp. | Eye phenotypes | Smed-sFRP-1 Expression | Brain/CG presence (3C11, Smed-GluR) |
Smed-prep(RNAi) trunks, 12dR | 11 | 0%, 2 eyes 92%, 1 eye (389/424) 18%, no eye (35/424) | 32% (8/25)*1 | 3c11 84% (27/32)*1 GluR 89% (33/37)*1 |
Smed-prep(RNAi) tails 12dR | 11 | 0%, 2 eyes 58%, 1 eye (241/417) 42%, no eye (176/417) | 0% (22/22) | 3c11 47% (15/32)*1 GluR 51% (18/35)*1 |
Smed-prep(RNAi) in regenerating tails, 12dR | 2 | 0%, 2 eyes 19%, 1 eye (4/26) 81%, no eye (21/26) | 0% (9/9) | |
Smed-prep(RNAi), lateral regeneration, 15dR | 2 | 36%, 2 eyes (9/25) 56%, 1 eye (14/25) 8%, no eye (2/25) | 3c11 100% (9/9) GluR 100% (11/11) | |
gfp(RNAi) summary, 12dR, 15dR | 15 | 100%, 2 eyes (350/351) 0%, 1 eye 0%, no eye (1/351) | 100% (32/32) | 3c11 100% (35/35) GluR 100% (42/42) |
Smed-prep(RNAi) intact animals, 28d+ homeostasis | 3 | 86%, 4 eyes (24/28) 14%, 2 eyes (4/28) | 100% (6/6)*2 | 3c11 100% (8/8) GluR 100% (9/9) |
Smed-prep/gfp(RNAi), 12dR | 3 | 17% (1/6)*1 | GluR 75% (9/12) | |
Smed-ndk/gfp(RNAi), 12dR | 3 | Ectopic eyes present | 100% (16/16) | GluR 100% (14/14)*3 |
Smed-ndk/prep(RNAi), 12dR | 3 | Ectopic eyes present | 10% (2/20)*1 | GluR 100% (15/15)*3 |
Smed-gfp(RNAi), 12dR | 3 | 100% (15/15) | GluR 100% (15/15) |
*1 Strongly reduced expression.
*2 All retain some very weak expression in the longitudinal double row of cells; the normally far stronger expression along the anterior head margin is completely absent.
*3 Posteriorly expanded expression.