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. 2010 Apr 22;6(4):e1000915. doi: 10.1371/journal.pgen.1000915

Table 1. Summary of phenotypes for Smed-prep(RNAi) experiments.

Experiment Nr. Exp. Eye phenotypes Smed-sFRP-1 Expression Brain/CG presence (3C11, Smed-GluR)
Smed-prep(RNAi) trunks, 12dR 11 0%, 2 eyes 92%, 1 eye (389/424) 18%, no eye (35/424) 32% (8/25)*1 3c11 84% (27/32)*1 GluR 89% (33/37)*1
Smed-prep(RNAi) tails 12dR 11 0%, 2 eyes 58%, 1 eye (241/417) 42%, no eye (176/417) 0% (22/22) 3c11 47% (15/32)*1 GluR 51% (18/35)*1
Smed-prep(RNAi) in regenerating tails, 12dR 2 0%, 2 eyes 19%, 1 eye (4/26) 81%, no eye (21/26) 0% (9/9)
Smed-prep(RNAi), lateral regeneration, 15dR 2 36%, 2 eyes (9/25) 56%, 1 eye (14/25) 8%, no eye (2/25) 3c11 100% (9/9) GluR 100% (11/11)
gfp(RNAi) summary, 12dR, 15dR 15 100%, 2 eyes (350/351) 0%, 1 eye 0%, no eye (1/351) 100% (32/32) 3c11 100% (35/35) GluR 100% (42/42)
Smed-prep(RNAi) intact animals, 28d+ homeostasis 3 86%, 4 eyes (24/28) 14%, 2 eyes (4/28) 100% (6/6)*2 3c11 100% (8/8) GluR 100% (9/9)
Smed-prep/gfp(RNAi), 12dR 3 17% (1/6)*1 GluR 75% (9/12)
Smed-ndk/gfp(RNAi), 12dR 3 Ectopic eyes present 100% (16/16) GluR 100% (14/14)*3
Smed-ndk/prep(RNAi), 12dR 3 Ectopic eyes present 10% (2/20)*1 GluR 100% (15/15)*3
Smed-gfp(RNAi), 12dR 3 100% (15/15) GluR 100% (15/15)

*1 Strongly reduced expression.

*2 All retain some very weak expression in the longitudinal double row of cells; the normally far stronger expression along the anterior head margin is completely absent.

*3 Posteriorly expanded expression.