Figure 4.

Agonist high affinity binding: autoradiography of brain slices from WT and VGV/Y mice. Representative [¹²⁵I]-DOI autoradiography. (A) WT sections incubated in 0.14 nM [¹²⁵I]-DOI plus 1 μM spiperone. (B) VGV/Y sections incubated in 0.14 nM [¹²⁵I]-DOI plus 1 μM spiperone. (C) WT sections incubated in 0.14 nM [¹²⁵I]-DOI plus 100 μM GTPγS. (D) VGV/Y sections incubated in 0.14 nM [¹²⁵I]-DOI plus 100 μM GTPγS. The addition of GTPγS reduced binding in choroid plexus of sections from WT, but not VGV/Y mice.