Skip to main content
. 2009 Sep 30;3:49. doi: 10.3389/neuro.22.001.2009

Figure 1.

Figure 1

Neural precursor cells in their local microenvironment in vivo and in vitro. (A) Immunohistochemical analysis of a hippocampal section from mouse showing the close association of neural precursor cells with neurons. Confocal image (optical section of 1 μm thickness). Green: Nestin-GFP; Red: Dcx, Blue: NeuN; Scale bar, 25 μm. (B) Confocal projections (56×) of the dentate gyrus (z-stack of 11 optical sections with 2 μm thickness); Green: Nestin-GFP; Red: Dcx; Blue: NeuN; one square in the underlying grid equals 16.4 μm. (C) Calretinin-immunohistochemistry highlights the inner molecular layer. Radial glia-like type-1 cells (Green) reach into this area, which receives the input from the entorhinal cortex. Green: NestinEGFP; Red: Calretinin; Blue: NeuN. (D) Newborn neurons in the adult dentate gyrus are in close neighborhood with neurites in the mouse hippocampus; Green: NestinEGFP, Red: MAP2ab, Blue: Dcx. (E) Representative image of precursor cells differentiated into neurons in co-culture of precursor cells with primary mouse hippocampal neurons. EGFP cells differentiated into neurons and also non-neuronal cells types, presumably glial cell, in co-culture. Green: EGFP (precursor cells); Red: βIII-tubulin (neurons). (F) The neurons generated from precursor cells in co-culture integrate into the underlying neuronal circuit as suggested by the presence of synapses (arrows) onto it indicated by the synaptophysin staining. Green: EGFP; Red: βIII-tubulin, Blue: synaptophysin. Scale bar [in (F) for (C–F)]: C, 250 μm; D, 200 μm; E, 25 μm; F, 50 μm.