Fig. 1.

Menin specifically binds to the caspase 8 locus in vivo. Menin-null or menin-expressing MEFs were seeded at 1×10⁶ cells per 100mm dish on day 0. On day 1, cells were processed to cross-link the DNA/protein complexes, which were incubated with anti-menin antibody or rabbit IgG control. On day 2, DNA/complexes were precipitated with protein A agarose beads, followed by reverse-crosslinking. Purified DNA was used for PCR templates with 0.1% input as positive controls. A pair of primers covering 5’-UTR, +208 to +623 from transcription start site (TSS), were used for PCR amplification