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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Dev Cell. 2010 Feb 16;18(2):288–299. doi: 10.1016/j.devcel.2009.12.012

Figure 2. kbr is a negative regulator of imaginal disc growth.

Figure 2

(A–B) Scanning electron micrographs (SEM) of a wildtype eye (A) and an eye composed predominantly of kbr mutant cells (B). Note the increased eye size in (B). Genotypes: (A) y w ey-flp; FRT82B/FRT82B Ubi-GFP, (B) y w ey-flp; FRT82B kbrdel/FRT82B Ubi-GFP.

(C–D) Same as in (A–B) except that stereomicroscopic images are shown. Note that adult eyes mosaic for kbr (D) contained predominantly mutant tissues (white), whereas eyes mosaic for a control chromosome contained far less white tissues (C).

(E–F) SEM images of fly eyes in which kbr was overexpressed by the GMR-Gal4 driver. Genotypes: (E) GMR-Gal4/UAS-kbr, (F) GMR-Gal4 UAS-kbr/UAS-kbr.

(G–H) TUNEL staining of wildtype (G) and GMR-Gal4 UAS-kbr/UAS-kbr (H) eye imaginal discs. Note the ectopic cell death in kbr-overexpressing eye disc (H).

(I–I’’’) A mid-pupal retina containing kbr mutant clones, marked by the lack of GFP (I), and stained for Discs-Large (Dlg, I’) and DAPI (I’’’). Superimposed GFP and Dlg are shown in (I’’). Note the increased number of interommatidial cells in kbr clones.

See Figure S2 for supplemental data to Figure 2.