Figure 2.
Activity-induced changes in the number of BrdU-labeled cells and new neurons in the dentate gyrus. (A,B) Confocal projections of the dentate gyrus (z-stack of 10 optical sections with 1.7-μm thickness). BrdU, Red; DCX, Blue. Scale bar, 25 μm in (A–D) Confocal image (optical section of 1-μm thickness) of STDSTD in (C) and RUNENR in (D) showing NeuN, Green; BrdU, Red, S100β, Blue; Inset: a, NeuN; b, BrdU; c, S100β. (D) Arrowheads in red indicating colocalization of BrdU and NeuN. Scale bar, 25 μm in (C,D). (E–H) Brightfield images of BrdU-positive cells in the dentate gyrus. Scale bar (in E for E–H), 100 μm.