Table 1.
Effect of RNAi with different silencer constructs in HEK293 cells.
| Time | ||||
|---|---|---|---|---|
| 48 h | 72 h | |||
| Silencer constructs | G/R ratio (%) | n | G/R ratio (%) | n |
| pSiU6-Ctrl | 100.0 ± 2.6 | 283 | 100.0 ± 2.6 | 308 |
| pSiU6-shGFP-1 | 35.3 ± 1.1 | 298 | 24.3 ± 1.4 | 198 |
| pSiU6-shGFP-2 | 17.8 ± 0.7 | 290 | 13.4 ± 0.9 | 170 |
| pSiU6-shGFP-3 | 15.1. ± 1.1 | 250 | 8.5 ± 0.4 | 168 |
| pSiEnU6-Ctrl | 100.0 ± 2.2 | 300 | 100.0 ± 4.4 | 241 |
| pSiEnU6-shGFP-1 | 32.7 ± 1.2 | 263 | 21.8 ± 1.1 | 187 |
| pSiEnU6-shGFP-2 | 16.5 ± 0.6 | 328 | 16.4 ± 1.1 | 232 |
| pSiEnU6-shGFP-3 | 17.0 ± 0.8 | 254 | 10.5 ± 0.6 | 168 |
| pSiH1-Ctrl | 100.0 ± 2.9 | 173 | 100.0 ± 2.6 | 208 |
| pSiH1-shGFP-1 | 35.9 ± 1.5 | 192 | 22.4 ± 1.3 | 173 |
| pSiH1-shGFP-2 | 18.2 ± 0.9 | 183 | 17.0 ± 0.9 | 168 |
| pSiH1-shGFP-3 | 13.4 ± 0.6 | 168 | 12.8 ± 0.7 | 193 |
| pSiEnU6-Ctrl CMV-Ago1 | 100.0 ± 2.0 | 239 | 100.0 ± 1.9 | 234 |
| pSiEnU6-shGFP-1 CMV-Ago1 | 44.8 ± 1.3 | 210 | 56.7 ± 1.9 | 145 |
| pSiEnU6-shGFP-2 CMV-Ago1 | 22.0 ± 0.7 | 230 | 23.6 ± 1.1 | 153 |
| pSiEnU6-shGFP-3 CMV-Ago1 | 31.6 ± 0.9 | 241 | 41.1 ± 2.3 | 175 |
| pSiEnU6-Ctrl CMV-Ago2 | 100.0 ± 1.4 | 260 | 100.0 ± 1.5 | 237 |
| pSiEnU6-shGFP-1 CMV-Ago2 | 19.8 ± 1.2 | 320 | 9.9 ± 0.9 | 150 |
| pSiEnU6-shGFP-2 CMV-Ago2 | 4.6 ± 0.4 | 224 | 3.4 ± 0.2 | 175 |
| pSiEnU6-shGFP-3 CMV-Ago2 | 8.9 ± 0.5 | 245 | 6.6 ± 0.3 | 244 |
Note: Data for pSiEnU6-shGFPs, pSiEnU6-shGFPs CMV-Ago1 and pSiEnU6-shGFPs CMV-Ago2 are from an experiment independent from the data plotted in Figure 3 and Table 2. G/R ratio: green to red fluorescence intensity ratio normalized to controls, which contain no shGFP expression in the same pSi configuration. n, number of cell examined.